Zebron ZB-BAC-1 GC Columns
Fast, Accurate Blood Alcohol Analysis
- Proprietary column set specially designed for accurate blood alcohol analysis — enhance resolution of ethanol and acetone peaks
- Resolves t-butanol and n-propanol for greater selection of internal standards
- Fast run time with baseline resolution of key components in just 2 minutes
- Achieve dual column confirmation - get two elution order changes with ZB-BAC-2
Need an alternate selectivity for blood alcohol confirmation? See ZB-BAC-2.
 
Testing drugs of abuse? See ZB-Drug-1.
Recommended Use
Abused Inhalant Anesthetics, Blood Alcohol Analysis
Recommended Use Enhanced resolution of ethanol and acetone peaks for blood alcohol analysis and dual-column confirmation with two elution order changes when used with ZB-BAC-2 |
Upgrade to Zebron from these similar* phases:
| Agilent® | Restek® |
|---|---|
| DB®-ALC1 DB-ALC2 |
Rtx®-BAC1 Rtx-BAC2 Rtx-VMS |
*not exact equivalent, selectivity may differ
Faster, More Sensitive Blood Alcohol Analysis
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Column:
As listed
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Dimension:
As listed
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Injection:
Split 0.8:1 @ 150 °C, 1 mL
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Carrier Gas:
Helium @ 80 cm/sec (constant flow)
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Oven Program:
40 °C (Isothermal)
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Detector:
FID @ 250 °C |
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Sample:
Analytes 0.025 % and internal standards 0.100 % in water 1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. t-Butanol (IS) 7. n-Propanol (IS) 8. 2-Butanol (IS) |
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Column:
As listed
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Dimension:
As listed
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Part No.:
7HK-G021-36 (ZB-BAC-1)
7HK-G022-32 (ZB-BAC-2) |
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Injection:
Split 5:1 @ 150 °C, 1 mL
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Carrier Gas:
Helium @ 80 cm/sec (constant flow)
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Oven Program:
40 °C (Isothermal)
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Detector:
FID @ 220 °C |
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Sample:
Analytes and internal standards 0.100 % in water 1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. t-Butanol (IS) 7. n-Propanol (IS) 8. 2-Butanol (IS) |
In contrast, the Phenomenex Zebron ZB-BAC-1 & and ZB-BAC-2 columns were able to fully resolve both t-Butanol and 2-Butanol from other target analytes, while maintaining rapid analysis times. Quantitative analysis on the Zebron BAC pair showed equivalent performance between all three internal standards (n-Propanol, t-Butanol, and 2-Butanol). The LOD & and LOQ for this column set were far below the control limits required by current testing regulations.
Estimated Limits of Detection (LOD) and Quantitation (LOQ) on Zebron ZB-BAC1 and ZB-BAC2 phases. The LODs and LOQs are also displayed in units of ppm in addition to % blood alcohol content.
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Compound |
Zebron ZB-BAC1 |
Zebron ZB-BAC2 |
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|---|---|---|---|---|
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LOD |
LOQ |
LOD |
LOQ |
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Methanol |
0.0003 % |
0.001 % |
0.0003 % |
0.001 % |
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Acetaldehyde |
0.00002 % |
0.00008 % |
0.00002 % |
0.00006 % |
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Ethanol |
0.0003 % |
0.0009 % |
0.0002 % |
0.0007 % |
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Isopropanol |
0.0002 % |
0.0006 % |
0.0001 % |
0.0005 % |
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Acetone |
0.00008 % |
0.0003 % |
0.00005 % |
0.0002 % |
High reproducibility of Zebron ZB-BAC1 and ZB-BAC2 for blood alcohols. Relative RSD values for replicate injections (n=5) were taken at the lowest concentration in the calibration curve of 0.025 %.
Zebron ZB-BAC-1
Compound |
Peak Area % RSD (Absolute) |
Peak Area % RSD (Relative to t-Butanol) |
Peak Area % RSD (Relative to n-Propanol) |
Peak Area % RSD (Relative to 2-Butanol) |
|---|---|---|---|---|
Methanol |
3.1 |
2.8 |
2.7 |
2.6 |
Acetaldehyde |
2.1 |
1.6 |
1.5 |
1.4 |
Ethanol |
3.4 |
2.8 |
2.7 |
2.6 |
Isopropanol |
3.2 |
2.6 |
2.5 |
2.4 |
Acetone |
2.2 |
1.6 |
1.5 |
1.4 |
Zebron ZB-BAC-2
Compound |
Peak Area % RSD (Absolute) |
Peak Area % RSD (Relative to t-Butanol) |
Peak Area % RSD (Relative to n-Propanol) |
Peak Area % RSD (Relative to 2-Butanol) |
|---|---|---|---|---|
Methanol |
2.1 |
1.6 |
1.4 |
1.3 |
Acetaldehyde |
3.1 |
2.7 |
2.5 |
2.4 |
Ethanol |
3.2 |
2.7 |
2.5 |
2.4 |
Isopropanol |
2.2 |
1.6 |
1.4 |
1.3 |
Acetone |
3.2 |
2.7 |
2.5 |
2.4 |
