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Clarity SPE

Product Information
Brand
Explore ClaritySPE™ oligonucleotide purification products by Phenomenex for efficient extraction and reliable, reproducible results in your analyses. Read more.

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

  • Works for desalting and clean up of biological samples
  • Specially formulated buffer solutions available for optimal performance
  • Quick protocols for rapid LC-MS/MS results
  • No liquid-liquid extractions (LLE)

Clarity Sample Preparation Products for Synthetic DNA/RNA
clarity-package

Oligonucleotide Purification Products

Clarity OTX™ SPE

Clarity OTX™ Extraction Kits

Prior to LC-MS analysis, therapeutic oligonucleotides must be isolated from interferences such as salts, sugars, large proteins, and genomic DNA. Using a mixed-mode solid phase extraction (SPE) sorbent in conjunction with carefully formulated buffers, Clarity OTX consistently delivers high recoveries.

 

Clarity QSP™ SPE

Clarity QSP™ High-Throughput Purification

Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.

 

Clarity RP-Desalting™

Clarity RP-Desalting™ Quick Salt Removal

Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.

 

Material Characteristics

Clarity Products Particle Support Bonded Phase Particle Shape/Size (μm) Pore Size(Å) Surface Area (m2/g)

Clarity OTX™ SPE

Polymer (surface modified PSDVB)

Mixed-mode anion exchanger

33

85

800

Clarity QSP™ SPE

Polymer (PSDVB)

Hydrophilic polymer coating

35

500

300

Clarity RP-Desalting™

Silica

C18

55

140

300

Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145

Clarity BioSolutions Product Selection

Purification

  Clarity Oligo-RP™ LC Columns Clarity Oligo-MS™ LC Columns
Clarity Oligo-XT LC Columns
Clarity OTX™ LC Columns
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Characterization / Analysis

  Clarity QSP™ Clarity Oligo-RP™ LC Columns
Clarity Oligo-XT LC Columns
Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
purification
RP-HPLC purification of failure
sequences from target sequences
Quick removal or salt & excess reagent
Purities >90% >90% ~70%
Recoveries ~90% ~70% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified