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Phase Detail
Zebron ZB-FAME GC Columns
Zebron ZB-FAME GC columns is designed for fatty acid methyl ester analysi
Zebron ZB-FAME GC Columns
Zebron ZB-FAME GC Columns
The Fast FAME GC Column
- Reduce run times for traditional FAMEs separations up to 75 %
- Separate cis/trans FAME isomers with Rs ≥ 1.0
- High-cyanopropyl selectivity acceptable for use with AOCS, AOAC, and IOC methods
Application Notes:
- Demonstration of Robustness and Selectivity for the Analysis of FAMEs in Salmon Oil and Pet Food. Read more.
Recommended Use
Fatty acid methyl esters (FAMEs), cis/trans FAME isomers, Omega 3 and Omega 6 FAMES
Recommended Use Resolution of fatty acid methyl esters (FAMEs) and cis/trans FAME isomers |
Upgrade to Zebron from any high cyanopropyl phase:
| Agilent® | Supelco® |
|---|---|
| CP-Sil 88 HP-88 DB®-23 |
SP®-2380 SP-2560 |
Accelerating FAME Testing
Designed to move beyond the conventional to the exceptional , Zebron GC columns come to life through a coupling of innovative spirit and technical excellence. As part of the Zebron Unlimited series of columns, Zebron ZB FAME offers unparalleled performance through the power of targeted selectivity when analyzing fatty acid methyl esters (FAMEs)
Optimized High-Cyano Polarity
FAME separations require the use of high polarity columns for adequate resolution of cis/trans isomers. Zebron™ ZB-FAME is an optimized high-cyanopropyl (G48) chemistry that provides the polarity that you need, while remaining compatible with AOAC, OCS, and IOC regulatory methods.
Optimized High-Cyano Polarity
FAME separations require the use of high polarity columns for adequate resolution of cis/trans isomers. Zebron™ ZB-FAME is an optimized high-cyanopropyl (G48) chemistry that provides the polarity that you need, while remaining compatible with AOAC, OCS, and IOC regulatory methods.
Elution Order for 37 FAME Standard
|
Peak
|
Compound
|
ID
|
|---|---|---|
|
1
|
Butanoic Acid Methyl Ester
|
C4:0
|
|
2
|
Hexanoic Acid Methyl Ester
|
C6:0
|
|
3
|
Octanoic Acid Methyl Ester
|
C8:0
|
|
4
|
Decanoic Acid Methyl Ester
|
C10:0
|
|
5
|
Undecanoic Acid Methyl Ester
|
C11:0
|
|
6
|
Dodecanoic Acid Methyl Ester
|
C12:0
|
|
7
|
Tridecanoic Acid Methyl Ester
|
C13:0
|
|
8
|
Myristic Acid Methyl Ester
|
C14:0
|
|
9
|
Myristoleic Acid Methyl Ester
|
C14:1 cis 9
|
|
10
|
Pentadecanoic Acid Methyl Ester
|
C15:0
|
|
11
|
cis-10-Pentadecenoic Acid Methyl Ester
|
C15:1 cis 10
|
|
12
|
Hexadecanoic Acid Methyl Ester
|
C16:0
|
|
13
|
Palmitoleic Acid Methyl Ester
|
C16:1 cis 9
|
|
14
|
Heptadecanoic Acid Methyl Ester
|
C17:0
|
|
15
|
cis-10-Heptadecenoic Acid Methyl Ester
|
C17:1 cis 10
|
|
16
|
Stearic Acid Methyl Ester
|
C18:0
|
|
17
|
Elaidic Acid Methyl Ester
|
C18:1 trans 9
|
|
18
|
Oleic Acid Methyl Ester
|
C18:1 cis 9
|
|
19
|
Linolelaidic Acid Methyl Ester
|
C18:2 trans 9,12
|
|
20
|
Linoleic Acid Methyl Ester
|
C18:2 cis 9,12
|
|
Peak
|
Compound
|
ID
|
|---|---|---|
| 21 |
γ-Linolenic Acid Methyl Ester
|
C18:3 cis 6,9,12
|
|
22
|
α-Linolenic Acid Methyl Ester
|
C18:3 cis 9,12,15
|
|
23
|
Arachidic Acid Methyl Ester
|
C20:0
|
|
24
|
cis-11-Eicosenoic Acid Methyl Ester
|
C20:1 cis 11
|
|
25
|
cis-11,14-Eicosadienoic Acid Methyl Ester
|
C20:2 cis 11,14
|
|
26
|
Heneicosanoic Acid Methyl Ester
|
C21:0
|
|
27
|
cis-8,11,14-Eicosatrienoic Acid Methyl Ester
|
C20:3 cis 8,11,14
|
|
28
|
Arachidonic Acid Methyl Ester
|
C20:4 cis 5,8,11,14
|
|
29
|
cis-11,14,17-Eicosatrienoic Acid Methyl Ester
|
C20:3 cis 11,14,17
|
|
30
|
Behenic Acid Methyl Ester
|
C22:0
|
|
31
|
Erucic Acid Methyl Ester
|
C22:1 cis 13
|
|
32
|
cis-5,8,11,14,17-Eicosapentaenoic Acid Methyl Ester
|
C20:5 cis 5,8,11,14,17
|
|
33
|
cis-13,16-Docosadienoic Acid Methyl Ester
|
C22:2 cis 13,16
|
|
34
|
Tricosanoic Acid Methyl Ester
|
C23:0
|
|
35
|
Lignoceric Acid Methyl Ester
|
C24:0
|
|
36
|
Nervonic Acid Methyl Ester
|
C24:1 cis 15
|
|
37
|
cis-4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester
|
C22:6 cis 4,7,10,13,16,19
|
QC Tested For FAMEs
Baseline Separation of Common Isomers
Food and dietary standards require separation of cis/trans fatty acids. Zebron™ ZB-FAME is able to separate from C4 to C24 with resolution of specific cis/trans fatty acids.
Completely Resolve AOAC Critical Pairs
|
Columns:
Zebron ZB-FAME
|
|
|
Dimension:
30 meter x 0.25 mm x 0.20 µm
|
|
|
Part No.:
7HG-G033-10
|
|
|
Injection:
Split 50:1 @ 240 °C, 1 µL
|
|
|
Recommended Liner:
Zebron PLUS Single Taper with Wool |
|
|
Liner Part No.:
AG2-0A11-05 (for Agilent® systems) |
|
|
Carrier Gas:
Helium @ 1.2 mL/min (constant flow)
|
|
|
Oven Program:
100 °C for 2 min to 140 °C @ 10 °C/min
to 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min |
|
|
Detector:
FID @ 260 °C |
|
|
Sample:
37 FAME standard |
Separate Detailed C18 Compounds
Due to regulatory guidelines around trans fat content in food, detailed analysis of specific FAMEs isomers is commonly performed. The below methods demonstrate this testing using a 60 meter Zebron™ ZB-FAME column.
Analysis of C18:1, C18:2, and C18:3
|
Columns:
Zebron ZB-FAME
|
|
|
Dimension:
60 meter x 0.25 mm x 0.20 µm
|
|
|
Part No.:
7KG-G033-10
|
|
|
Injection:
Split 50:1 @ 240 °C, 1 µL
|
|
|
Recommended Liner:
Zebron PLUS Single Taper with Wool |
|
|
Liner Part No.:
AG2-0A11-05 (for Agilent® systems) |
|
|
Carrier Gas:
Helium @ 30 cm/sec (constant flow)
|
|
|
Oven Program:
160 °C (isothermal)
|
|
|
Detector:
FID @ 260 °C |
|
|
Sample:
As listed. For full peak identification, visit www.phenomenex.com/DetailedFAME |
Twice The Runs, Half The Time
Traditionally, cis/trans FAME separations require the use of long (100 meters or more) columns and can run up to 60 minutes, resulting in a bottleneck to higher productivity. Zebron™ ZB-FAME provides targeted selectivity that allows for reduced column length – run times as short as 11 minutes without compromising your results!
37 FAMEs In A Short 11 Minute Run
|
Columns:
Zebron ZB-FAME
|
|
|
Dimension:
20 meter x 0.18 mm x 0.15 μm
|
|
|
Part No.:
7FD-G033-05
|
|
|
Injection:
Split 100:1 @ 250 °C, 1 µL
|
|
|
Recommended Linear:
Zebron PLUS Single Taper Z-Liner™ |
|
|
Liner Part No.:
AG2-0A13-05 (for Agilent® systems) |
|
|
Carrier Gas:
Helium @ 1.0 mL/min (constant flow)
|
|
|
Oven Program:
80 °C for 1.5 min to 160 °C @ 40 °C/min to
185 °C @ 5 °C/min to 260 °C @ 30 °C/min
|
|
|
Detector:
FID @ 260 °C |
|
|
Sample:
37 FAME standard |
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