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Product Information
Phase Detail
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns is packed with an 8% cross-linked hydrogen ion exclusion media
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns
- 8% cross-linked hydrogen
- For use with organic acids in combination with carbohydrates, alcohols, fatty acids, neutral compounds; amino sugars
- Uniquely suited to monitor starches, sugars, organic acids, and ethanol in one run for bioethanol fermentation analysis
- Achieve excellent base line separation between Dp3 and Dp4+
Particle Size 8 |
Recommended Use Organic acids alone, organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds, ethanol, acetic acid, glycerol, standard alcohol mixtures, and amino sugars |
| Rezex | Description | Applications | Bio-Rad® Aminex® | Supelco® Supelcogel™ | Waters® Sugar-Pak™ | Transgenomic® CARBOSep™ | Sepax® Carbomi® |
|---|---|---|---|---|---|---|---|
| ROA-Organic Acid (USP L22 packing)* | 8 % cross-linked HYDROGEN | – Organic acids alone – Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds – Ethanol, acetic acid, glycerol, and standard alcohol mixtures – Amino sugars |
HPX-87H 125-0140 |
Supelcogel C-610H & H |
N/A | N/A | N/A |
Sharper Peak Shape = Easy & Accurate Quantitation
Organic Acids in Wine
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Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
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Dimension:
300 x 7.8 mm
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Mobile Phase:
0.005 N H2SO4
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Flow Rate:
0.5 mL/min
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Temperature:
40 °C
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Detection:
UV @ 210 nm
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Sample:
1. Citric acid
2. Tartaric acid 3. Succinic acid 4. Lactic acid 5. Acetic acid |
Lower Backpressure = Longer Column Lifetimes
and Faster Run Time Capability
and Faster Run Time Capability
Aliphatic Acids
Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.
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Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
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Dimension:
300 x 7.8 mm
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Mobile Phase:
0.005 N H2SO4
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Flow Rate:
0.5 mL/min
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Temperature:
40 °C
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Detection:
UV @ 210 nm
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Sample:
1. Oxalic acid
2. Citric acid 3. Tartaric acid 4. Succinic acid 5. Formic acid 6. Acetic acid |
Food and Beverage
White Wine
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Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
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Dimension:
300 x 7.8 mm
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Part No.:
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Mobile Phase:
0.005 N H2SO4
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Flow Rate:
0.5 mL/min
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Temperature:
40 °C
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Detection:
UV @ 210 nm
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Sample:
1. Oxalic acid
2. Citric acid 3. Tartaric acid 4. Succinic acid 5. Formic acid 6. Acetic acid |
Bioethanol
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Column:
Rezex ROA-Organic Acid
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Dimension:
300 x 7.8 mm
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Part No.:
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Mobile Phase:
0.005 N Sulfuric Acid
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Flow Rate:
0.6 mL/min
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Temperature:
60 °C
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System:
RI @ 40 °C
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Detection:
Shimadzu® Prominence® LC-20A System
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Sample:
1. Dp4+
2. Dp3 3. Maltose 4. Glucose 5. Lactic Acid 6. Glycerol 7. Acetic Acid 8. Ethanol |
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Column:
Rezex ROA-Organic Acid
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Dimension:
150 x 7.8 mm
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Part No.:
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Mobile Phase:
0.005 N Sulfuric Acid
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Flow Rate:
0.6 mL/min
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Temperature:
60 °C
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System:
RI @ 40 °C
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Detection:
Shimadzu® Prominence® LC-20A System
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Sample:
1. Dp4+
2. Dp3 3. Maltose 4. Glucose 5. Lactic Acid 6. Glycerol 7. Acetic Acid 8. Ethanol |
Saccharides
Monosaccharides with Organic Acids
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Column:
Rezex ROA-Organic Acid
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Dimension:
300 x 7.8 mm
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Part No.:
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Mobile Phase:
0.1 % formic acid
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Flow Rate:
0.5 mL/min
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Temperature:
75 °C
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System:
ELSD
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Sample:
1. Citric acid
2. Tartaric acid 3. Glucose 4. Fructose 5. Succinic acid |
Alcohols
Carboxylic Acids
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Column:
Rezex ROA-Organic Acid
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Dimension:
300 x 7.8 mm
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Part No.:
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Mobile Phase:
Water + 0.5 % Trifluoroacetic Acid
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Flow Rate:
1.0 mL/min
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Temperature:
40 °C
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System:
RI
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Sample:
1. Acetylene Carboxylic Acid
2. Maleic Acid 3. Succinic Acid 4. Fumaric Acid |
Monosaccharides with Organic Acids
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Column:
Rezex ROA-Organic Acid
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Dimension:
300 x 7.8 mm
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Part No.:
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Mobile Phase:
1 % Phosphoric Acid
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Flow Rate:
0.6 mL/min
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Temperature:
Ambient
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System:
RI
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Sample:
1. Glucose
2. N-Acetylglucose 3. N-Acetylgalactosamine |
Applications (partial list)
Call us for assistance in selecting the right column for your needs!
| Compound | App ID No. |
|---|---|
| Acetic Acid | 5515 |
| Acetylene Carboxylic Acid | 5517 |
| Ethanol | 5512 |
| Formic Acid | 5512 |
| Fumaric Acid | 5517 |
| Glucose | 5514 |
| Isopropanol | 5512 |
| Maleic Acid | 5517 |
| Compound | App ID No. |
|---|---|
| Methanol | 5512 |
| n-Butanol | 5512 |
| n-Propanol | 5512 |
| n-Acetylgalactosamine | 5514 |
| n-Acetylglucose | 5514 |
| Oxalic Acid | 5515 |
| sec-Butanol | 5512 |
| Succinic Acid | 5515 |
Specifications and Operating Recommendations
| ROA Organic Acid |   |
|---|---|
| Part No. | 00H-0138-K0 |
| Ionic Form | Hydrogen |
| Standard Dimensions | 300 x 7.8 mm |
| Matrix | Sulfonated Styrene Divinyl Benzene |
| Cross Linking | 8 % |
| Particle Size (μm) | 8 |
| Min. Efficiency (p/m) based on last peak | 50,000 (Acetic Acid) |
| Typical Pressure (psi @ Testing Flow Rate) | 580 |
| Max. Pressure (psi @ Max Flow Rate) | 1,000 |
| Max. Flow Rate (mL/min) | 1.0 |
| Max. Temperature (°C) | 85 |
| Typical Mobile Phase | 0.005 N H2SO4 |
| pH Range | 1-8 |
| Guard Column Part | 03B-0138-K0 |
| Cleaning, Regeneration and Storage |   |
|---|---|
| Organic Modifiers (Max) | 5 % Methanol, IPA, EtOH |
| Inorganic Modifiers | 5 % HNO3, H3PO4 |
| Avoid | Acids, Bases, Salts or Metal Ions, pH > 3, > 30 % Acetonitrile |
| Cleaning Solvent | 100 % Water |
| Flow Rate(mL/min) | 0.4 |
| Temperature (°C) | 85 |
| Duration (hrs) | 12 |
| Regeneration Solvent | 0.025 M H2SO4 |
| Flow Rate (mL/min) | 0.2 |
| Duration (hrs) | 4-16 |
| Ship/Storage Solvent | 0.005 N H2SO4 |
Cross Reference Guide
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