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Phase Detail
Luna C18(2) HPLC Columns
Discover Phenomenex Luna C18(2) HPLC columns with ultra-high purity silica, exceptional reproducibility, and scalability for all your chromatography needs.
Luna C18(2) HPLC Columns
Luna C18(2) HPLC Columns
Discover the unmatched performance of the Luna C18(2) column, designed for consistent, high-quality chromatographic results.
The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with a very hydrophobic stationary phase that offers great methylene selectivity
- Extensive line of rugged particle sizes
- Easy method scalability from HPLC to preparative and bulk purification LC
- C18(2) Ultra pure, metal-free silica (99.99% purity) (USP:L1)
Carbon Load 17.5, 17.5 % |
Surface Area (m²/g) 400 |
Particle Size 2.5, 3, 5, 10, 15 |
Recommended Use Recommended for improved methylene selectivity for USP methods or general LC method development |
Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the back-bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.
Superior Particle Smoothness
Agilent Technologies® ZORBAX® 5 µm SB-C18
Waters® Symmetry® 5 µm C18
Phenomenex Luna 5 µm C18
The high efficiencies and bonded phase surface coverage provide for sharp peaks The C8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.
Peak Asymmetry Comparison
Conditions for all columns:
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Dimension:
150 x 4.6 mm
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Mobile Phase:
Acetonitrile/Water (50:50)
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Flow Rate:
1.0 mL/min
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Temperature:
Ambient
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Detection:
UV @ 254 nm
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Sample:
1. Pyridine
2. Phenol
3. Toluene
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The high efficiencies and bonded phase surface coverage provide for sharp peaks Whether you need a column for USP methods or just general method development the Luna C18(2) column should be your first choice every time.
Polar, Acidic Drugs
Conditions for all columns:
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Columns:
 Phenomenex Luna 3 µm C18(2) Waters® Symmetry® 3.5 µm C18 |
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Dimension:
75 x 4.6 mm
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Mobile Phase:
20 mM KH2PO4/ Acetonitrile (70:30)
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Flow Rate:
0.75 mL/min
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Detection:
UV @ 202 nm
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Sample:
1. Tolmetin
2. Naproxen
3. Diflunisal
4. Fenoprofen
5. Indomethacin
6. Ibuprofen
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Hydrophobic, Acidic Compounds
Conditions for all columns:
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Columns:
Phenomenex Luna® 5 µm C18(2) Thermo Hypersil-Keystone® HyPURITY™ Elite 5 µm C18 |
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Dimension:
150 x 4.6 mm
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Mobile Phase:
20 mM Potassium phosphate, pH 2.5 / Acetonitrile (75:25)
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Flow Rate:
1.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 254 nm
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Sample:
1. p-Hydroxybenzoic acid
2. Sorbic acid*
3. Benzoic acid*
4. Salicylic acid
5. p-Toluic acid
* Sorbic acid and Benzoic acid co-elute on HyPURITY Elite
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Basic Compounds
Comparative separations may not be representative of all applications.
Conditions for all columns:
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Columns:
Phenomenex Luna® 5 µm C18(2)
Macherey-Nagel® Nucleosil® 5 µm C18 |
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Dimension:
150 x 4.6 mm
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Mobile Phase:
20 mM Potassium phosphate,
pH 2.5/Acetonitrile (75:25)
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Flow Rate:
1.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 210 nm
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Sample:
1. Maleic acid
2. Triprolidine*
3. Chlorpheniramine*
4. Diphenhydramine*
*Peaks 2-4 adsorb on Nucleosil C18
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