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Product Information
Phase Detail
Clarity Oligo-XT
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations.
Clarity Oligo-XT
Clarity Oligo-XT
A Sensitive Solution for Oligo Characterization and Bioanalysis
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies, making Clarity Oligo-XT a great choice for analyses that require sensitivity such as oligo characterization and oligo analysis from bioanalytical samples.
Particle Size 1.7, 2.6, 5 |
Recommended Use Rapid, high efficiency RP-LC-MS from purification to characterization |
LC-MS Analysis
Oligo-XT
An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times as compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results.Clarity Core-Shell
Conventional Fully Porous
The Core-Shell Advantage
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.
| Fully Porous |
Clarity Core-Shell |
Average Efficiency Gain with Clarity* |
|
|---|---|---|---|
 |
VS. |  |
90 % Higher |
 |
VS. |  |
85 % Higher |
 |
VS. |  |
20 % Higher |
Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
*May not be representative of all applications.
Sensitive, Reliable Analysis
Calibration curve of BNA in mini pig liver homogenate
Column:
Clarity 5 μm Oligo-XT
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Dimension:
50 x 2.1 mm
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Part No.:
00B-4745-AN
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HPLC System:
Shimadzu® Nexera® X2 UHPLC
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Mobile Phase:
A: 1.0 % HFIP & 0.1 % DIEA
with 10 μM EDTA in Methanol B: 1.0 % HFIP & 0.1 % DIEA with 10 μM EDTA in Methanol / Water (50:50 v/v) |
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Gradient:
Time (min)
0.5
2.5 3.0 3.5 4.0 5.0 | % B
30 60 100 100 30 30 |
Flow Rate:
500 μL/min |
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Temperature:
40 ËšC |
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Detection:
Thermo Q Exactiveâ„¢ Hybrid
Quadrupole-Orbitrapâ„¢ Mass Spectrometer, HESI, negative polarity |
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Injection Volume:
10 μL |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide