What Parameters Should I Consider When Selecting a GC Column for My Application?

Polarity: Some indication of polarity will usually be provided by the GC column manufacturer. The value provided is normally determined using Kovats retention time indices together with McReynold’s numbers. The general advice is that stationary phase polarity should closely mirror the polarity of the analytes of interest, so a non-polar phase such as a ZB-1 (rated as 5 on the polarity scale) would be a good choice for hydrocarbon analysis, whilst a ZB-Wax (57 on the polarity scale) is often chosen for alcohols.

Selectivity: This is controlled by the different functional groups present within the polymeric stationary phase, the base of which is either polysiloxane or polyethylene glycol. Polysiloxane phases will typically have methyl groups, phenyl groups or cyanopropyl groups attached to the polysiloxane chains which allow for a variety of different interactions to occur:

• Methyl groups – van der waals or London forces
• Phenyl groups – pi-pi interactions
• Cyano groups – pi-pi and dipole-dipole interactions

Increasing the percentage of phenyl or cyanopropyl substituents in the stationary phase increases polarity. Polyethylene glycol (PEG) phases offer sites for dipole-dipole and hydrogen bonding interactions to take place. PEG phases are typically some of the most polar phases available.

Length: Increasing the length of the column increases the number of theoretical plates, which aids resolution, however, increasing column length also increases the run time of any analysis conducted. A 30 m column is generally a good starting point for general method development, whereas 60 m columns are typically used for the fingerprint analysis of complex mixtures. 15 m columns are generally utilised when the rapid separation of less complex mixtures is required.

ID: The choice of column ID impacts both column capacity (how much analyte you can inject) and efficiency. The narrower the column ID, the higher the efficiency but the lower its capacity. A good place to start with method development is a 0.25 mm ID column, which show a good compromise between efficiency and capacity. If you are converting a method from an old-style packed GC column to capillary GC columns, then 0.53 mm ID columns are often chosen due to their higher analyte capacity.

Film thickness: Thin films provide the highest efficiency but have lower analyte retention. Thicker films have a higher capacity but lead to more bleed at high temperatures. If choosing to use a thin film (0.1 - 0.25 µm) with active analytes, it is preferential to select a column which is highly deactivated to prevent peak tailing. Such columns generally provide lower bleed too.